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Autoantibody-producing B cells, having the potential to be targeted for immunotherapy of autoimmune diseases, were originally identified by in vitro phage antibody display technology. These cells can be easily enriched from peripheral blood mononuclear cells by in vivo selection using an immunodeficient mouse model. Unfortunately, autoreactive cells produced in this manner have not been well defined. To characterize the autoantibodies, cells should be separated from background Ig from the original animal, so that their antigenic specificity is made known. Here, we describe protocols to generate a mouse model with a T cell-dependent autoimmune system and to enrich autoantibody-producing B cells with the help of helper T cells. A lethal irradiation and bone marrow transplantation strategy is used for generating mouse models. Serum antibodies against an antigen of interest can be generated from animals with serological evidence of immune system reconstitution. We used this model for the identification of an autoantibody-producing cell in a mouse model.{
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“description”: “JavaScript UI Kit: Beta”,
“fork”: false,
“full_name”: “cristaljimenez/TruWaDE”,
“language”: “JavaScript”,
“updated_at”: “2015-02-27T23:42:34.188423”
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