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In summary, we validated the specificity and sensitivity of the ORF3-TES antibody. We also demonstrated that the membrane-bound epitope of PrP^C^ undergoes proteolytic cleavage during infection, which is useful in screening for PrP^Sc^. The membrane-bound form of PrP^C^ in our model confirmed the proposed co-translational cleavage of PrP^C^, which could be helpful in understanding the mechanism of the membrane-bound PrP^C^ generation. The association between PrP^Sc^ and PrP^C^ was also investigated using the ORF3-TES antibody and an in vivo model. The findings suggested that the formation of the PrP^C^-PrP^Sc^ complex is based on the direct membrane-bound interaction of PrP^C^ and PrP^Sc^, not on the indirect interaction via the host cell. The results also indicated that the structure of the transmembrane region of PrP^C^ is probably affected by the formation of the complex.
This work was supported by the National Natural Science
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